Journal
FOOD CHEMISTRY
Volume 114, Issue 4, Pages 1576-1582Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2008.11.097
Keywords
2-furfural; 5-Methylfurfural; 5-Hydroxymethylfurfural; Patulin; Honey; White and demerara sugars; Balsamic vinegar; Caramel; Nutritional supplements; Apple juices; HPLC; Markers of quality
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This work describes an improved, simple high performance liquid chromatography (HPLC) method for the simultaneous determination of furfuraldeydes (2-furfural (24), 5-methylfurfural (5-MF) and 5-hydroxymethylfurfural (5-HMF)) and patulin in food products. The compounds were simultaneously determined by reversed-phase HPLC using a C-18 Column and photodiode array (DAD) detection. The method, simple and accurate, showed recoveries of furfuraldeydes from 6% to 100% and the recovery of patulin is about 90%. The method was applied to different commercial food matrices: honey. white and demerara table sugars. white and red balsamic vinegars, caramel, nutritional supplement, sugar substitute, apple juices (clear and cloudy) and also to a mould infected apple. The one-step, sensitive (the limits of detection (LODs) for patulin, 5-HMF, 5-MF and 2-F were 0.09, 0.06, 0.23 and 0.73 ppb, respectively), no time consuming methodology will contribute to characterize and quantify the presence of these compounds in human diet and to determine the quality of foods. (C) 2008 Elsevier Ltd. All rights reserved.
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