Isolation and characterisation of the isoflavones from sprouted chickpea seeds

Seven isoflavones were isolated from sprouted chickpea seeds by chromatography on silica gel column, polyamide column, sephadex LH-20 column and preparing thin-layer chromatography (TLC), respectively. The structures were characterised with one- and two-dimensional NMR in combination with mass and IR spectrometry. The obtained isoflavones were biochanin A (5,7-dihydroxyflavone-4'-methoxyflavone, (1): calycosin (7,3'-dihydroxy-4'-methoxyisoflavone, (2): formononetin (7-hydroxy-4'-methoxyisoflavone, (3); genistein (5,7,4'-trihydroxyisoflavone, (4); trifolirhizin (maackiain-3-O-beta-D-glucopyranoside, (5); ononin(7-O-beta-D-glucosyl-7-hydroxy-4'-methoxyisoflavone, (6): sissotrin (7-O-beta-D-glucosyl-5,7-dihydroxy-4'-methoxyisoflavone, (7). (C) 2008 Published by Elsevier Ltd.