Journal
FOOD CHEMISTRY
Volume 113, Issue 4, Pages 1101-1105Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2008.08.078
Keywords
Seaweeds; Rhodomela confervoides; Antioxidant activity; Phenolic content; DPPH; beta-Carotene-linoleate; Reducing power
Funding
- National High-Tech R D Project [2007AA09Z403]
- National Natural Science Foundation of China [30530080]
- Department of Science and Technology of Shandong Province [2006GG2205023]
- Qingdao Municipal Science and Technology Commission for award [06-2-2-12-JCH]
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The methanol-chloroform extract of the marine red alga, Rhodomela confervoides, was measured for antioxidant activity, using the alpha,alpha-diphenyl-beta-picrylhydrazyl radical-scavenging assay and the beta-carotene-linoleate bleaching assay systems, and compared with those of the positive Controls of butylated hydroxytoluene, gallic acid and ascorbic acid, The active extract was further purified by liquid-liquid partition to afford four fractions, of which the ethyl acetate-soluble (EA) fraction exhibited the strongest antioxidant activity in both assay systems. This fraction was further divided into seven subfractions, designated as EA1-EA7, by silica gel vacuum liquid chromatography. in most cases, EA1 and EM Were found to possess the strongest activity. The total phenolic contents and reducing powers of the extract, fractions, and subfractions were also determined. Significant associations between the antioxidant potency and the total phenolic content, as well as between the antioxidant potency and the reducing power, were found for the tested fractions and subfractions. (c) 2008 Elsevier Ltd. All rights reserved.
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