Journal
FOOD CHEMISTRY
Volume 114, Issue 3, Pages 844-851Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2008.10.083
Keywords
Atlantic salmon; Coho salmon; Alaska pollack; Southern blue whiting; Hydrolysis; Size exclusion chromatography; ACE inhibitory activity; DPPH radical scavenging activity
Funding
- Nippon Suisan Kaisha Ltd. (Tokyo, Japan)
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To utilise Atlantic salmon, Cohn, salmon, Alaska pollack, and southern blue whiting as components of neutraceutical food and to clarify the potential physiological function of those fishes, their muscles were hydrolysed with pepsin, pancreatin or thermolysin. Methanolic extracts of fish muscle and their hydrolysates were prepared for analysis of angiotensin converting enzyme (ACE) inhibitory and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities. Pepsin hydrolysates of all the fish samples did not increase degree of hydrolysis, extractive nitrogen content and bioactivities. Pancreatin and thermolysin improved the ACE inhibitory activity, and the activity was expressed following the peak of size exclusion chromatography (SEC). The DPPH radical scavenging activity was increased following pancreatin and thermolysin hydrolysis, but this activity was not related to the SEC result. Except for the lower DPPH radical scavenging activity of Alaska pollack pancreatin hydrolysate than those of the others, there was no significant difference in the ACE inhibitory and DPPH radical scavenging activities by fish species. (C) 2008 Elsevier Ltd. All rights reserved.
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