Journal
FOOD CHEMISTRY
Volume 113, Issue 4, Pages 1113-1118Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2008.08.084
Keywords
DNA; Carotenoids; Cisplatin; Reactive oxygen species; Chemiluminescence
Funding
- Brazilian Agency Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [150164/2005-0]
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This work evaluated the Modulation of reactive oxygen species (ROS) produced by the cisplatin-human DNA interaction in a cell-free experimental model by the carotenoids bixin and lycopene extracted from, natural dietary Sources and purified through luminol- and Cypridina luciferin methoxy-analogue (MCLA)- enhanced chemiluminescence assays. The results showed that the ROS generation by DNA-cisplatin interaction was inhibited by both lycopene and bixin in a concentration-dependent manner. At a concentration of 100 mu M, lycopene and bixin inhibited Superoxide anion (O center dot(2)) generation at 90% and 82%, respectively, and the total ROS generation at 44% and 42%, respectively. The formation of significant amounts of isomers or degradation products of both carotenoids was not observed after ROS scavenging, as evaluated by high-performance liquid chromatography. Taken together, these results Suggest that carotenoids can be helpful to Modulate the oxidative stress found in cancer therapy with cisplatin. (c) 2008 Elsevier Ltd. All rights reserved.
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