Journal
FOOD CHEMISTRY
Volume 112, Issue 1, Pages 232-238Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2008.05.105
Keywords
Topas 19/2; genetically modified organism; herbicide-tolerant rapeseed; event-specific method; real-time PCR; quantitative PCR
Funding
- Special Foundation of Social Benefits of China [2005DIA2J026]
- National High Technology Research and Development Program of China [2006AA10Z444]
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The herbicide-tolerant transgenic rapeseed Topas 19/2 (synonym HCN92) has been approved for environmental release in Canada, Japan, Australia and the USA, and exported to a number of other countries as raw material. The purpose of this study was to establish event-specific qualitative and quantitative detection methods for Topas 19/2. The T-integration junction sequence spanning the host plant DNA and the integrated transgene of the Topas 19/2 event was isolated and identified. The event-specific qualitative detection method was established to produce an amplicon of 110 basepairs (bp) with an absolute detection limit of 10 initial template copies. The event-specific quantitative detection method was developed with the limit of detection (LOD) and limit of quantification (LOQ) being approximately 5 and 50 initial template copies, respectively. The developed real-time PCR systems were assessed using two mixed rapeseed samples with known Topas 19/2 contents. Expected results were obtained. Crown Copyright (c) 2008 Published by Elsevier Ltd. All rights reserved.
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