In vitro and in vivo antioxidant activity of Choerospondias axillaris aqueous extract from fruit

In the present study, an aqueous extract from Choerospondias axillaries fruit was evaluated for its in vivo antioxidant activity, using the D-galactose induced mouse aging model, and for its in vitro scavenging effects on the superoxide anions, DPPH, H2O2, OH center dot. The reducing power and Fe (2+)-chelating ability, as well as the inhibition of lipid peroxidation were also evaluated. The flavonoid and phenolic contents of the extract were determined. Pertaining to the in vivo activity, the intragastric administration of the extract inhibited D-galactose induced oxidative damage. Furthermore, in the in vitro assays, the extract showed a high antioxidant effect, especially scavenging of DPPH anions and its reducing power. The total content of phenolic and flavonoid compounds was 568 mg of gallic acid equivalents/g dry material and 2.09 mg of quercetin equivalents/g dry material respectively. These results provide scientific support for the empirical use of C axillaries fruit as a medicine for cardiovascular diseases. (C) 2007 Published by Elsevier Ltd.