Journal
FOOD CHEMISTRY
Volume 107, Issue 1, Pages 352-361Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2007.08.027
Keywords
Citrus depressa; pectin; high-methoxyl; structural characterisation; endocarp
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A polysaccharide was extracted with hot HCl from the endocarp of Citrus depressa grown in Okinawa, Japan. The yield was 4.1% on a fresh weight basis. Composition of the polysaccharide was 89.3% total carbohydrate, 79.2% uronic acid, 4.1% ash and 8.8% moisture. The degree of methoxylation was estimated to be 66.2%. The polysaccharide was composed of D-GalA, D-Gal, L-Ara and L-Rha in the ratio of 100:10.3:1.53:0.94, respectively. The molecular mass was estimated to be approximately 4.1 x 10(4). NMR spectra indicated that the polysaccharide was mainly composed of (1 -> 4)-linked alpha-D-GalA and (1 -> 4)-linked beta-D-Gal. Methylation analysis results identified 1 -> 2 and 1 -> 2,4 linked Rha, 1 -> 4, 1 -> 3, 1 -> 6 and 1 -> 3,6 linked Gal. The results indicated that the polysaccharide was a pectin, which was relatively simpler than other pectins. The polysaccharide was classified as high-methoxyl pectin. The pectin turned into a gel by the generalised method. (c) 2007 Elsevier Ltd. All rights reserved.
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