Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 74, Issue -, Pages 91-97Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2014.09.003
Keywords
Sodium selenite; Aflatoxin B 1; Bursa of Fabricius; Apoptosis; Immunoglobulin; Broiler
Categories
Funding
- Program for Changjiang Scholars
- University Innovative Research Team [IRT 0848]
- Science and Technology Department of Sichuan Province [2012FZ0066]
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To investigate the effects of sodium selenite against aflatoxin B1 (AFB 1), 200 male Avian broilers, divided into five groups, were fed with basal diet (control group), 0.3 mg/kg AFB1 (AFB1 group), 0.3 mg/kg AFB1 + 0.2 mg/kg Se (+Se group I), 03 mg/kg AFB1 +0.4 mg/kg Se (+Se group II) and 03 mg/kg AFB1 +0.6 mg/kg Se (+Se group III), respectively. Compared with the control group, decreased relative weight of bursa of Fabricius and contents of serum immunoglobulin, more vacuoles and debris in the bursal lymphoid follicle, and increased percentage of apoptotic bursal cells were observed in the AFB1 group. Sodium selenite, however, could increase the relative weight of bursa of Fabricius and contents of serum immunoglobulin, and ameliorate histopathological lesions. The percentages of apoptotic bursal cells, through flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling method, in the three +Se groups were lower than those in the AFB I group. Compared with the AFB 1 group, moreover, the mRNA expressions of Bax and Caspase-3 by qRT-PCR in the three +Se groups were decreased, while the expression of Bcl-2 was increased. The results indicate that sodium selenite in diet can protect chicken from AFB 1-induced impairment of humoral immune function by reducing bursal histopathological lesions and percentages of apoptotic bursal cells. (C) 2014 Elsevier Ltd. All rights reserved.
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