Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 55, Issue -, Pages 484-492Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2013.01.039
Keywords
Cranberry; Polyphenols; Urinary metabolites; Ultra-performance liquid chromatography; Mass spectrometry
Categories
Funding
- Spanish Ministry of Economy and Competitiveness [AGL2011-29857-C03-02]
- Andalusian Regional Government Council of Innovation and Science for the Excellence [P09-CTS-4564, P10-FQM-6563, P11-CTS-7625]
- University of Andalas for the Directorate General of Higher Education(DGHE) scholarship [2609/D4.4/2008]
- [2661]
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As the beneficial effects of American cranberry (Vaccinium macrocarpon) can be partly attributed to its phenolic composition, the evaluation of the physiological behaviour of this fraction is crucial. A rapid and sensitive method by ultra-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) has been used to identify phenolic metabolites in human urine after a single dose of cranberry syrup. Prior to the analysis, metabolites were extracted using an optimised solid-phase extraction procedure. All possible metabolites were investigated based on retention time, accurate mass data and isotope and fragmentation patterns. Free coumaroyl hexose (isomer 1 and 2), dihydroxybenzoic acid, caffeoyl glucose, dihydroferulic acid 4-O-beta-D-glucuronide, methoxyquercetin 3-O-galactoside, scopoletin, myricetin and quercetin, together with other 23 phase-I and phase-II metabolites, including various isomers, could be tentatively identified in the urine. Afterwards, the metabolites were simultaneously screened in the urine of different subjects at 0, 2, 4, and 6 h after the ingestion of cranberry syrup by Target Analysis (TM) software. (C) 2013 Elsevier Ltd. All rights reserved.
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