4.7 Article

Dealcoholated red wine induces autophagic and apoptotic cell death in an osteosarcoma cell line

Journal

FOOD AND CHEMICAL TOXICOLOGY
Volume 60, Issue -, Pages 377-384

Publisher

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2013.07.078

Keywords

Red wine; Polyphenols; Apoptosis; Autophagy; U2Os cell line

Funding

  1. Italian Ministry of Economy and Finance [191/2009]
  2. FESR Campania Region
  3. MIUR (Italian Minister of Education, University and Scientific Research) [RBNE08ZZN7]
  4. Italian Ministry of Health/Istituto Zooprofilattico Sperimentale del Mezzogiorno

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Until recently, the supposed preventive effects of red wine against cardiovascular diseases, the so-called French Paradox, has been associated to its antioxidant properties. The interest in the anticancer capacity of polyphenols present in red wine strongly increased consequently to the enormous number of studies on resveratrol. In this study, using lyophilized red wine, we present evidence that its anticancer effect in a cellular model is mediated by apoptotic and autophagic cell death. Using a human osteosarcoma cell line, U2Os, we found that the lyophilized red wine was cytotoxic in a dose-dependent manner with a maximum effect in the range of 100-200 mu g/ml equivalents of gallic acid. A mixed phenotype of types I/II cell death was evidenced by means of specific assays following treatment of U2Os with lyophilized red wine, e.g., autophagy and apoptosis. We found that cell death induced by lyophilized red wine proceeded through a mechanism independent from its anti-oxidant activity and involving the inhibition of PI3K/Akt kinase signaling. Considering the relative low concentration of each single bioactive compound in lyophilized red wine, our study suggests the activation of synergistic mechanism able to inhibit growth in malignant cells. (C) 2013 Elsevier Ltd. All rights reserved.

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