Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 59, Issue -, Pages 470-475Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2013.06.044
Keywords
Aflatoxin B-1; Probiotics; AFB(1)-degrading enzyme; Broiler production performance; Gene expressions
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In order to degrade aflatoxin B-1 (AFB(1)), AFB(1)-degrading microbes (probiotics) such as Lactobacillus casei, Bacillus subtilis and Pichia anomala, and the AFB(1)-degrading enzyme from Aspergillus oryzae were selected and combined to make feed additive. Seventy-five 43-day-old male Arbor Acres broilers were randomly divided into 5 groups, 15 broilers for each group. The broilers were given with 5 kinds of diets such as the basal diet, 400 mu g/kg AFB(1) supplement without feed additive, and 200, 400, 800 mu g/kg AFB(1) supplement with 0.15% feed additive. The feeding experimental period was 30 d, which was used to determine production performance of broilers. In addition, serum, liver and chest muscle were selected for measuring AFB(1) residues, gene expressions, microscopic and antioxidant analyses. The results showed that adding 0.15% feed additive in broiler diets could significantly relieve the negative effect of AFB(1), on chicken's production performance and nutrient metabolic rates (P < 0.05). It could also improve AFB(1), metabolism, hepatic cell structure, antioxidant activity, and many hepatic enzyme gene expressions involved in oxidoreductase, apoptosis, cell growth, immune system and metabolic process (P < 0.05). It could be concluded that the feed additive was able to degrade AFB(1) and improve animal production. (C) 2013 Elsevier Ltd. All rights reserved.
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