Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 50, Issue 9, Pages 3273-3279Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2012.06.022
Keywords
RANKL; Sargachromanol G; Osteoclastogenic factor; MAPK; NF-kappa B
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Funding
- Korea Ocean Research and Development Institute [PE98592]
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Bone diseases are characterized by the presence of pro-inflammatory cytokines that regulate bone turnover. The receptor activator of NF-kappa B ligand (RANKL) is a soluble osteoblast-derived protein that induces bone resorption through osteoclast differentiation and activation. Sargachromanol G (SG) was isolated from the brown algae Sargassum siliquastrum; SG has anti-osteoclastogenic activity, but its mechanism of action and its active components remain largely unknown. In the present study, we investigated the anti-osteoclastogenic effects of SG on the expression of interleukin-1 beta (IL-1 beta)-induced osteoclastogenic factors (PGE(2), COX-2, IL-6, OPG, and RANKL) in the human osteoblast cell line MG-63. We also examined the role of the nuclear factor-kappa B (NF-kappa B) and the mitogen-activated protein kinase (MAPK) signaling pathways in IL-1 beta-stimulated MG-63 cells. SG dose-dependently inhibited the production of osteoclastogenic factors in MG-63 cells. SG also inhibited phosphorylation of MAPK (ERK1/2, p38, and JNK) and NF-kappa B (p65, p50, and I kappa B-alpha). These results suggest that the anti-osteoporotic effect of SG may be because of the modulation of osteoclastogenic factors via suppression of MAPK and NF-kappa B activation. (C) 2012 Elsevier Ltd. All rights reserved.
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