Inhibitory effect of Pleurotus eryngii extracts on the activities of allergic mediators in antigen-stimulated mast cells

Pleurotus eryngii is an edible mushroom native to Europe, the Middle East, and North Africa, and is also grown in parts of Asia. The present study investigated the anti-allergy potential of P. eryngii extract (PEE) in antigen-stimulated RBL-2H3 mast cells. PEE inhibited allergy markers, including release of hexosaminidase and histamine, in antigen-sensitized RBL-2H3 cells. PEE also suppressed the expression and production of interleukin-4 and reduced antigen-induced NFAT and NF-kappa B transcriptional activity in antigen-sensitized mast cells. Moreover, PEE decreased the levels of proinflammatory cytokines and COX-2 and iNOS expression in antigen-sensitized mast cells. Finally. PEE suppressed antigen-induced signal protein phosphorylation of Lyn, PLC gamma 2, PKC, Akt, and MAP kinases. Taken together, these results suggest that P. eryngii extract may provide insight into the prevention and treatment of allergic and inflammatory diseases. (C) 2011 Elsevier Ltd. All rights reserved.