Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 48, Issue 8-9, Pages 2319-2325Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2010.05.066
Keywords
Antrodia camphorata; Nuclear factor-kappa B; Lipopolysaccharide; Transgenic mice; Anti-inflammation
Categories
Funding
- National Science Council [NSC 96-2628-B-039-030-NY3]
- Committee on Chinese Medicine and Pharmacy, Department of Health [CCMP 97-RD-201]
- China Medical University [CMU97-190, CMU96-207, CMU97-CMC-004]
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In an earlier study, we found that Antrodia camphorata inhibited the production of lipopolysaccharide (LPS)-induced cytokines, inducible nitric oxide synthase, and cyclooxygenase-2 by blocking nuclear factor-kappa B (NF-kappa B) activation in cultured RAW 264.7 macrophages. This study was aimed at evaluating the inhibitory effects of the fermented culture broth of A. camphorata in terms of LPS-induced NF-kappa B activation in transgenic mice by using a non-invasive, real-time NF-kappa B bioluminescence imaging technique. Transgenic mice carrying the luciferase gene under the control of NF-kappa B were given A. cam phorata (570 mg/kg, p.o.) for three consecutive days and then injected with LPS (4 mg/kg, i.p.). In vivo imaging showed that treatment with LPS increased the luminescent signal, whereas A. cam phorata suppressed the LPS-induced inflammatory response significantly. Ex vivo imaging showed that A. camphorata suppressed LPS-induced NF-kappa B activity in the small intestine, mesenteric lymph nodes, liver, spleen, and kidney. Immunohistochemical staining revealed that A. camphorata suppressed production of the LPS-induced tumour necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and NF-kappa B p65 subunit in these organs. Furthermore, A. camphorata attenuated the productions of LPS-induced TNF-alpha and IL-1 beta in serum from transgenic mice. We report the first confirmation of the anti-inflammatory action in vivo of this potentially beneficial mushroom. (C) 2010 Elsevier Ltd. All rights reserved.
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