Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 48, Issue 1, Pages 53-60Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2009.09.014
Keywords
Aluminium phosphide; Cytochrome oxidase; Lipid peroxidation; Oxidative phosphorylation
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Funding
- Indian Council of Medical Research (ICMR), New Delhi, India
- Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India
- Council of Scientific and Industrial Research (CSIR), New Delhi, India
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The present study was designed with an aim to analyze the effect of acute aluminium phosphide (ALP) exposure (10 mg/kg b.wt, intragastrically) on the kinetic characteristics of cytochrome oxidase and energy metabolism in male Wistar rat liver mitochondria. Liver mitochondrial preparations from ALP-treated rats exhibited significant decrease (66%) in the activity of cytorchrome oxidase suggesting that there was a decrease in the catalytic efficiency of the active oxidase molecules on ALP treatment. The decreased activity of cytochrome oxidase with altered NADH and succinic dehydrogenase activities might have contributed towards a significant decline in state 3 and state 4 respiration as observed. These alterations in the electron transport chain complexes in turn adversely affected the ATP synthesis rate as well as ATP levels in the mitochondria isolated from treated rats. The alterations in the respiratory chain, was followed by enhanced lipid peroxidation in rat liver mitochondria which might have further contributed to change in the fluidity of membrane as depicted by decreased fatty acid content of liver mitochondria. However, no significant change was observed in cholesterol and phospholipids content in our study. The present study thus highlights the significance of altered mitochondrial respiratory chain functions and membrane integrity after acute ALP exposure. (C) 2009 Elsevier Ltd. All rights reserved.
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