Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 47, Issue 9, Pages 2389-2395Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2009.06.035
Keywords
Piper gaudichaudianum; Essential oil; Cytotoxicity; Genotoxicity; Mutagenicity; Lipid peroxidation
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Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Fundacao de Amparo a Pesquisa do Rio Grande do Sul (FAPERGS) [06/1979.5]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
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We have investigated the chemical composition of Piper gaudichaudianum essential oil, as well as its cytotoxic, mutagenic and genotoxic effects in V79 cells. The chemical analyses showed that the major compounds are (E)-nerolidol (22.4%), alpha-humulene (16.5%), (E)-caryophyllene (8.9%) and bicyclogermacrene (7.4%). Dose-dependent cytotoxic effects were observed in V79 cells treated with essential oil by using clonal survival, 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide reduction assay (MTT) and trypan blue exclusion assay (TB), and a significant decrease in survival was observed at concentrations of 0.5 mu g/mL and higher. The P. gaudichaudianum essential oil treatment caused DNA strand breaks in V79 cells at concentrations up to 2 mu g/mL, as detected by the alkaline comet assay, but did not induce double-strand breaks, as verified by neutral comet assay. It induced a significant increase in the frequency of micronucleated cells at 4, 6 and 10 mu g/mL Moreover, A gaudichaudianum essential oil significantly increased lipid peroxidation at doses of 0.5 mu g/mL and higher, suggesting that the observed oxidant potential can be responsible, at least in part, for its cytotoxic and genotoxic effects. (C) 2009 Elsevier Ltd. All rights reserved.
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