Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 46, Issue 7, Pages 2365-2368Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2008.03.018
Keywords
ABTS; antioxidant; fluorescein; free radical; ORAC; total antioxidant capacity
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Sub-additivity of antioxidant activities in assays of total antioxidant capacity has been reported previously and ascribed to binding of low-molecular weight antioxidants such as flavonoids by proteins. We demonstrate that this phenomenon is much more common and concerns also interactions between typical low-molecular weight oxidants in the assays of ABTS(.-) decolorization and protection of fluorescein from AAPH-induced bleaching. The subadditive interactions between antioxidants may affect quantitative considerations drawn from in vitro assays of antioxidant capacity of biological samples. (c) 2008 Elsevier Ltd. All rights reserved.
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