Journal
FOOD AND BIOPRODUCTS PROCESSING
Volume 88, Issue C1, Pages 61-66Publisher
INST CHEMICAL ENGINEERS
DOI: 10.1016/j.fbp.2009.06.006
Keywords
Aeration rate; Harvesting time; Particle size; Response surface methodology; Rice bran; Trichoderma reesei QM9414
Funding
- Scientific Center of Excellence for Recycling and Losses of Strategic Agricultural Products in Tarbiat Modares University (Tehran, Iran)
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In the present study, the effect of culture conditions on cellulase activities by Trichoderma reesei QM9414 grown on rice bran media in solid-state fermentation was evaluated. Also, the optimum conditions were determined using response surface methodology (RSM). A face center design was used to investigate the effect of independent variables i.e. particle size, aeration rate and harvesting time on filter paper activity (EPA), Avicelase and carboxy methyl cellulase (CMCase) activities. The highest FPA, Avicelase and CMCase activities were 11.648, 99.756 and 94.210 U/g dry weight of substrate (DWS), respectively. The results showed that aeration rate and particle size had significant effects on the activity of cellulase on FPA. Also, particle size and harvesting time had significant effects on the activity of both Avicelase and CMCase. The reduction of particle size and harvesting time to 1-1.5 mm and 4 days, respectively increased cellulase activities. (C) 2009 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
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