Journal
FOOD AND BIOPRODUCTS PROCESSING
Volume 88, Issue C1, Pages 40-46Publisher
ELSEVIER
DOI: 10.1016/j.fbp.2009.12.002
Keywords
Aqueous two-phase systems; Partitioning; Recycling; Bovine serum albumin
Funding
- National Natural Science Foundation of China [20876089]
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The extraction and back-extraction of bovine serum albumin (BSA) have been studied by liquid-liquid extraction with poly(ethylene glycol) (PEG)/potassium citrate aqueous two-phase system (ATPS). In this work, the ATPS was examined with regard to the effects of PEG molecular weight (PEG 1000, 2000, 4000 and 6000), PEG and potassium citrate concentration, BSA concentration (C-BSA) and pH on BSA partition. The pH was found to have significant effects on BSA partition with low molecular weight PEG 1000. The yield of the BSA, 99%, was obtained in the top phase under the following conditions: 19% (w/w) PEG 1000, 20% (w/w) potassium citrate and 0.75 mg/g (BSA) at pH 7.0 and 30 degrees C. BSA can be re-extracted to a new citrate phase by decreasing the pH of the system with a 92% yield. The back-extraction not only separates the BSA from the polymer, but also allows the polymer to be recycled. The global yield (Y-e + Y-be) is up to 91%. (C) 2009 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
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