Antioxidant-Rich Extract from Dehydrated Seabuckthorn Berries by Supercritical Carbon Dioxide Extraction

Seabuckthorn (Hippophae rhamnoides L.) berry oil having high nutraceutical, cosmeceutical, and therapeutic activity has been extracted from dried seabuckthorn (SBT) whole berry powder using supercritical carbon dioxide (SC-CO2), a green process for extraction of bioactives. The SC-CO2 process was optimized using Box-Behnken design. Three SC-CO2 parameters namely extracting pressure, extracting temperature, and time of extraction were examined. The optimal SC-CO2 conditions were determined, and the quadratic response surfaces were drawn from the mathematical models. A maximum recovery of 85.12% tocopherol, 71.73% carotene, and an EC50 of 29.02 mg/ml (from DPPH assay) was obtained after SC-CO2 extraction at 44 A degrees C, 345 bar, and run time of 80 min. Use of methanol as an entrainer at 30% v/w of SBT berry at optimized conditions further increased the extraction efficiency and the potency. This extract can be used for varied nutraceutical, cosmeceutical, and pharmaceutical applications.