Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 26, Issue 3, Pages 317-324Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2014.914469
Keywords
EDC; nadifloxacin; chicken muscle; indirect competitive enzyme-linked immunosorbent assay (ELISA)
Funding
- Key Programmes from MOST [2012BAD29B04]
- MOF
- MOE [201310135, JUSRP51308A]
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A convenient and specific competitive enzyme-linked immunosorbent assay (ELISA) for nadifloxacin (NDF) was developed. The modified 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) method was employed to synthesise the artificial antigen of NDF. The indirect competitive ELISA developed to detect NDF has a concentration at 50% inhibition (IC50) of 0.72 ng/mL and a limit of detection of 0.04 ng/mL. This ELISA kit could be used as a screening method to detect and control the illegal content of NDF in food products. In this study, the kit was applied to NDF detection in chicken muscle and produced a satisfactory result with recovery rates from samples spiked with NDF of 92% (10 ng/mL) and 101% (50 ng/mL).
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