Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 23, Issue 1, Pages 69-82Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2011.604770
Keywords
cow's milk allergy; alpha-casein; beta-casein; hydrolysis; papain; ELISA; antigen; enzyme
Funding
- National Natural Science Foundation of China [30471224, 30871817]
- National Science and Technology Ministry of China [2006BAD27B04, 2006BAD04A06]
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The effects of pH, temperature, enzyme-to-substrate ratio and reaction time on the antigenicity of casein hydrolysates were investigated. Response surface methodology (RSM) was employed to optimise the reaction conditions. Enzymatic hydrolysis with papain could reduce the antigenicity of alpha-casein and beta-casein effectively and the reduction of antigenicity could be controlled by regulation of the reaction conditions. The model for optimal reaction conditions of a lower antigenicity of alpha-casein and beta-casein was established. Under the range of conditions studied, enzyme-to-substrate ratio had the most significant effects on the antigenicity of alpha-casein and beta-casein. The anti-alpha-casein IgG binding inhibition and anti-beta-casein binding inhibition were both significantly negatively related with the degree of hydrolysis (DH).
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