Journal
FOOD ANALYTICAL METHODS
Volume 7, Issue 3, Pages 690-696Publisher
SPRINGER
DOI: 10.1007/s12161-013-9723-4
Keywords
Bee pollen; Immunoaffinity column; Low-temperature fat precipitation; Aflatoxins; Ochratoxin A
Categories
Funding
- National Natural Science Foundation of China [31172273]
- Special Fund for Agro-scientific Research in the Public Interest: Mycotoxin Control [201203037]
Ask authors/readers for more resources
A simple and sensitive method has been developed for simultaneous determination of aflatoxins (AFB1, AFB2, AFG1, and AFG2) and ochratoxin A (OTA) in bee pollen. The analytes in the sample were extracted with a mixture of acetonitrile/water (60:40, v/v), using low temperature for fat precipitation, followed by immunoaffinity column cleanup of extracts. The mycotoxins were quantified using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with an electrospray ionization (ESI) and a triple quadrupole (QqQ) analyzer. Matrix effect, accuracy, and precision were evaluated and achieved good results calculated by matrix-matched calibration standards which reduced the influence of matrix effect. Recoveries at three levels were in the range of 74.3-96.5 % with RSD less than 10.0 %. The correlation coefficients (r (2)) of the five mycotoxins were higher than 0.997. The method showed high sensitivity with LOD below 0.05 mu g/kg.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available