4.4 Article

Evaluation of Dispersive Liquid-Liquid Microextraction-HPLC-UV for Determination of Deoxynivalenol (DON) in Wheat Flour

Journal

FOOD ANALYTICAL METHODS
Volume 6, Issue 1, Pages 176-180

Publisher

SPRINGER
DOI: 10.1007/s12161-012-9428-0

Keywords

Deoxynivalenol; DLLME; HPLC; Wheat flour

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A fast and simple method for the extraction of deoxynivalenol (DON) from wheat flour using dispersive liquid-liquid microextraction (DLLME) followed by high-performance liquid chromatography-UV detection has been developed and compared with immunoaffinity column cleanup (IAC) process. The influence of several important parameters on the extraction efficacy was studied. Under optimized conditions, a linear calibration curve was obtained in the range of 50-1,000 mu g/L. Average recoveries of DON from spiked wheat samples at levels of 500 mu g/kg for DLLME and IAC ranged from 72.9 +/- 1.6 and 85.5 +/- 3.1, respectively. A good correlation was found for spiked samples between DLLME and IAC methods. The limit of detection was 125 and 50 mu g/kg for DLLME and IAC method, respectively. Advantages of DLLME method with respect to the IAC have been pointed out.

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