Journal
FOOD ANALYTICAL METHODS
Volume 1, Issue 2, Pages 73-84Publisher
SPRINGER
DOI: 10.1007/s12161-008-9025-4
Keywords
Extraction; Concentration; PCR; Vegetables; Fruit; Norovirus; Hepatitis A Virus
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Virus-contaminated soft fruits or vegetables are increasingly identified as causes of foodborne viral illness. Noroviruses and hepatitis A virus are the most common pathogens in viral infections transmitted by these kinds of foods. To improve microbiological detection and monitoring and to increase insights into the contribution of fruits and vegetables to foodborne viral transmission, sensitive, reliable, and standardized methods are needed. More studies on virus detection methods for foods are being published, but validated consensus protocols are not yet available. In this paper, different methodologies are reviewed critically. The use of process controls and internal amplification controls is discussed.
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