Journal
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
Volume 30, Issue 2, Pages 389-394Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/19440049.2012.743042
Keywords
antibody; capillary zone electrophoresis; citrinin; fermented food; immunoaffinity column
Funding
- National Natural Science Foundation of China [21105058]
- China Postdoctoral Science Foundation [20100471569]
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An immunoaffnity column (IAC) for the clean-up of citrinin-contaminated food samples was prepared by using silica gel immobilised with a anti-citrinin antibody. Antibodies produced against citrinin-bovine serum albumin (BSA) were covalently immobilised onto a silica-based solid support to prepare the IAC. The selective extraction and clean-up ability of the IAC was evaluated by capillary zone electrophoresis coupled with ultraviolet detection. Clean-up conditions such as eluting solutions kind, concentrations, pH and volumes were optimised for citrinin by using the IAC. IACs were applied to citrinin-contaminated foods such as red yeast rice and monascus colour. The method's performance was acceptable in terms of recoveries, which ranged from 80.4% to 97.1% for citrinin-spiked samples at levels of 50, 100 and 200?mu g?kg-1, and the relative standard deviation ranged from 5.3% to 10.5%.
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