Journal
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
Volume 29, Issue 6, Pages 925-934Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/19440049.2012.662703
Keywords
HPLC; immunoassays; screening - ELISA; screening - immunoassays; residues; veterinary drug residues - antibiotics; animal products - meat
Funding
- Scientific and Technological Research Project of Chongqing China [CSTC2011ggB10009]
- Natural Science Foundation of Chongqing Education Committee [KJ110605]
- Chongqing Normal University
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A new method was developed for the determination of quinoxaline-2-carboxylic acid, the marker residue of carbadox, in the edible tissues of food-producing animals using a colloidal gold probe-based immunochromatographic assay. The highly specific polyclonal antibody (PcAb), which was very sensitive to N-butylquinoxaline-2-carboxylic acid (BQCA) with an IC50 value of 2.38 ng ml(-1), was selected for the development of an immunochromatographic assay (ICA). Only 5 min were required to perform this assay; it had a visual detection limit of 25 ng g(-1) for quinoxaline-2-carboxylic acid. The results of the analysis of quinoxaline-2-carboxylic acid in animal tissues using the immunochromatographic assay showed good agreement with those obtained by HPLC. In conclusion, the method was rapid and accurate for screening residues of carbadox in the edible tissues of food-producing animals.
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