Development of chemiluminescence enzyme-linked immunosorbent assay for the screening of metolcarb and carbaryl in orange juice, cabbage and cucumber

A direct competitive enzyme-linked immunosorbent assay (ELISA) with enhanced chemiluminescent detection for simultaneous detection of metolcarb and carbaryl in agricultural products is reported. The concentrations of coating antibodies and enzyme tracer, as well as the effects of pH and methanol concentrations, were investigated. Under optimum conditions, the IC50 of metolcarb and carbaryl were 4 and 0.3 mu g kg-1, respectively. Detection of metolcarb and carbaryl in fruit juices and vegetables was accomplished using simple, rapid and efficient extraction methods. Recoveries (n = 3) of spiked samples ranged 75-108% for metolcarb and 74-98% for carbaryl. Validation of the enhanced chemiluminescent immunosorbent assay was conducted by comparison of results with HPLC. The correlation between the data obtained using ELISA with enhanced chemiluminescent detection and HPLC was good (n = 9). Therefore, the enhanced chemiluminescent ELISA was suitable for quantitative simultaneously determination of metolcarb and carbaryl residue in agricultural products.