Journal
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
Volume 26, Issue 6, Pages 800-807Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/02652030902755283
Keywords
Sudan dyes; ELISA; chilli powder; egg yolk; ketchup
Funding
- Italian Ministry for Universities [2005030782_001]
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This study aimed at developing sensitive competitive enzyme-linked immunosorbent assays (ELISAs) for the banned Sudan dyes using polyclonal antibodies. Three different formats were developed and characterized in terms of sensitivity, selectivity and rapidity. A competitive indirect ELISA was developed, which showed an IC50 of 3.8 mu g l(-1). Two competitive direct ELISAs were also developed, in which the antibody was added before or simultaneously with the other reagents; the first showed an IC50 of 8.3 mu g l(-1) and the latter showed an IC50 of 4.9 mu g l(-1). Nevertheless, considering dilution of extracts which is needed to offset matrix interference, the limits of detection of the three formats were substantially the same (10 mu g kg(-1)). The antibodies in all three test formats were able to recognize Sudan I and partially Sudan II, III and IV; no cross-reactivity was observed with the five edible dyes. Twenty food samples, including chilli powder, paprika, ketchup, and egg, were extracted by a simple sample preparation and very limited dilution. Extracts were analyzed by the developed competitive direct ELISA with the simultaneous addition of reagents. A good correlation was observed (y = 1.19x-10.0, r(2) = 0.991, n = 20) when the data was compared with that obtained through a conventional HPLC method.
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