Survey of ochratoxin A in rice from Iran using affinity column cleanup and HPLC with fluorescence detection

This paper records the occurrence and levels of ochratoxin A (OTA) in rice using a HPLC technique preceded by an immunoaffinity clean-up step. The method was based on the extraction of finely ground rice sample with an acetonitrile/water (60 : 40, v/v) solution. Recovery was 98.9% while the limit of detection (LOD) was 0.2 ng g-1. A total of 182 rice samples were analyzed with a frequency of contamination of 6%. Levels of OTA in positive samples ranged 0.2-4.8 ng g-1, with an average contamination of all analyzed samples of 1.6 ng g-1.