Journal
FOLIA MICROBIOLOGICA
Volume 57, Issue 1, Pages 1-4Publisher
SPRINGER
DOI: 10.1007/s12223-011-0089-y
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Funding
- Polish Ministry of Science and Higher Education [N N310 304639]
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This study describes the development of a new colorimetric assay to determine aromatic amino acid aminotransferase (ArAT) activity. The assay is based on the transamination of l-tryptophan in the presence of 2-oxoglutarate, which yields indole-3-pyruvate (IPyA). The amount of IPyA formed was quantified by reaction with the Salkowski reagent. Optimized assay conditions are presented for ArAT isozymes isolated from Pseudomonas putida. For comparative purposes, ArAT activity was also determined by high-performance liquid chromatography. ArAT activity staining in polyacrylamide gels with the Salkowski reagent is also presented.
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