Journal
FLY
Volume 5, Issue 4, Pages 304-306Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/fly.5.4.17810
Keywords
nociception; nocifensive; TRP receptor; Drosophila larvae
Categories
Funding
- MRC (UK) [G0400580]
- Medical Research Council [G0400580] Funding Source: researchfish
- MRC [G0400580] Funding Source: UKRI
Ask authors/readers for more resources
We describe a novel thermosensitive escape behavior in Drosophila larvae and a simple assay to accurately define the response temperature. When a larva is placed in a droplet of water that is subsequently heated, a stereotypical escape response is robustly elicited at 29 degrees C. Larvae defective for the painless TRP receptor, or blocked in the function of class IV multi-dendritic sensory dendrites respond to this stimulus at reproducibly higher temperature (34 degrees C). The escape response has novel behavioral components and a lower temperature threshold in comparison with the responses to touch with a hot needle. Furthermore the assay minimizes operator bias that is present in current tests of thermosensitive nociception and generates a precise determination of temperature at the point of response. This response is highly reproducible and directly applicable to genetic and neural circuit analysis of a simple escape behavior.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available