Journal
FLY
Volume 4, Issue 1, Pages 12-17Publisher
LANDES BIOSCIENCE
DOI: 10.4161/fly.4.1.10995
Keywords
Drosophila; BEAF; insulator; gene targeting; homologous recombination
Categories
Funding
- NSF [0718898]
- Louisiana Board of Regents [LEQSF (2004-05)-RD-A-11]
- LSU
Ask authors/readers for more resources
Gene replacement by homologous recombination is a powerful technique for generating mutations in Drosophila. While using this technique for the BEAF gene, we encountered non-targeted lethal mutations on the target chromosome that complicated the analysis of the BEAF mutations until they were discovered and removed by meiotic recombination. Subsequent experiments indicated that the gene-targeting method leads to a modest but significant three-fold increase in the rate of production of non-targeted lethal mutations. It is important to be aware of this phenomenon when using this method.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available