Journal
JOURNAL OF THE NEUROLOGICAL SCIENCES
Volume 353, Issue 1-2, Pages 63-69Publisher
ELSEVIER
DOI: 10.1016/j.jns.2015.04.003
Keywords
Spinal cord trauma; Secondary injury; A-D myelomalacia; 8-Isoprostane; Canine
Categories
Funding
- State of Indiana through the Center for Paralysis Research at Purdue University
- Purdue College of Veterinary Medicine Small Animal Disease Research Grant
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Multiple biochemical and immunohistochemical tests were performed to elucidate the role of oxidative stress during ascending-descending (A-D) myelomalacia by comparing dogs with this progressive terminal condition to dogs with chronic, focal spinal cord injuries (SCIs) and controls without SCI. Dog with A-D myelomalacia exhibited increased biochemical markers for oxidative stress, including 8-isoprostane F2 alpha and acrolein, as well as decreased endogenous glutathione with greatest changes occurring at the lesion center. Inflammation, as evident by the concentration of CD18 + phagocytes and hemorrhagic necrosis, was also exacerbated in the lesion of A-D myelomalacic spinal cord compared to focal SCI. The greatest differences in oxidative stress occurred at the lesion center and diminished distally in both spinal cords with A-D myelomalacia and focal SCIs. The spatial progression and time course of A-D myelomalacia are consistent with the development of secondary injury post-SCI. Ascending-descending myelomalacia is proposed as a clinical model that may further the understanding of the role of oxidative stress during secondary injury. Our results indicate that the pathology of A-D myelomalacia is also similar to subacute progressive ascending myelopathy in humans, which is characterized by recurrent neurodegeneration of spinal cord post-injury. (C) 2015 Elsevier B.V. All rights reserved.
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