Journal
FITOTERAPIA
Volume 89, Issue -, Pages 74-79Publisher
ELSEVIER
DOI: 10.1016/j.fitote.2013.05.015
Keywords
Flavonol glycosides; Psidium guajava L. extract; Dipeptidyl-peptidase inhibition; Diabetes mellitus; Gastrointestinal absorption; HPLC separation
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Based on the traditional use in popular medicine, the effect of extracts from Psidium guajava L. leaves and of the main flavonol-glycoside components on dipeptidyl-peptidase IV (DP-IV), a key enzyme of blood glucose homoeostasis, has been investigated in-vitro. An ethanolic extract was prepared from dried, powdered leaves of guava and was found to contain seven main flavonol-glycosides, which were isolated by semipreparative HPLC and tested individually. The ethanolic guava leave extract was shown to exert a dose-dependent inhibition of DP-IV, with an IC50 of 380 mu g/ml test assay solution. Also the individual flavonol-glycosides inhibited DP-IV dose-dependently, with variations of the effects by a factor of 10, and an overall effect accounting for 100% of that observed for the total guava extract. The recovery of individual flavonol-glycosides in CaCo-2 epithelial cells, a model of gastrointestinal tract absorption, amounted to 2.3-5.3% of the amount available for absorption over 60 min at 37 degrees C. (c) 2013 Elsevier B.V. All rights reserved.
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