Journal
FITOTERAPIA
Volume 82, Issue 3, Pages 352-356Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.fitote.2010.11.005
Keywords
Rheum undulatum; Streptococcus mutans; Acid production; Biofilms
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Funding
- Ministry of Education, Science and Technology [2009-0071090]
- National Research Foundation of Korea [2009-0071090] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Rheum undulatum root has been used traditionally in Korea for the treatment of dental diseases. The purpose of this study was to separate a fraction from R. undulatum showing anti-acid production activity against Streptococcus mutans biofilms and identify the main components in that fraction. Methanol extract of R. undulatum root and its fractions were prepared. To select a fraction exhibiting anti-acid production activity, suspension glycolytic pH-drop assay was performed. Among the fractions tested, dichloromethane fraction exhibited the strongest activity in a dose-dependent manner. To examine the effect of the selected fraction on the anti-acid production of S. mutans biofilms, 74 h old S. mutans biofilms were used. The selected fraction reduced the initial rate of acid production of S. mutans biofilms at sub-minimum inhibitory concentration (MIC) levels. HPLC qualitative analysis of the selected fraction indicated that the presence of anthraquinone derivatives, such as aloe-emodin, emodin, chrysophanol and physcion, as main components. (c) 2010 Elsevier B.V. All rights reserved.
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