Journal
FITOTERAPIA
Volume 82, Issue 8, Pages 1160-1168Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.fitote.2011.07.014
Keywords
Traditional Chinese medicine; Synergistic effect; Hepatoprotective effect; Pharmacokinetics; PCA, principal component analysis
Categories
Funding
- Natural Science Foundation of the State [90709019]
- National Key Program on the Subject of Drug Innovation [2009ZX09502-005]
- National Program for Key Basic Research Projects in China [2005CB523406]
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6,7-Dimethylesculetin (D), geniposide (G) and rhein (R) are the three major active ingredients of Yin-Chen-Hao-Tang (YCHT), a famous Chinese herbal formula, which has been shown to be clinically effective for treating hepatic injury (HI) syndrome. The present study was conducted to investigate the therapeutic and synergistic effects of COC (combination of D. G and R) on HI rats by combining pharmacokinetic with biochemical analysis strategy. Plasma was analyzed by using reversed-phase high performance liquid chromatography (RP-HPLC). Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models were built to evaluate the therapeutic and synergistic effects of COC at the biochemical level. Here, we report that the COC combination could increase the plasma level, slow elimination rate, exert a more robust therapeutic effect than any one or two of the three individual compounds by hitting multiple targets in a rat model of HI. Overall, this beneficially accounts for the popular view that traditional Chinese medicine (TCM) formula usually takes multicomponent to exert their therapeutic effects. We suggest that dissecting the mode of action of clinically effective formula to be capable of producing a sufficient effect at low doses. (C) 2011 Elsevier B.V. All rights reserved.
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