Anti-inflammatory and analgesic properties of cis-mulberroside A from Ramulus mori

This study examined the analgesic and anti-inflammatory actions of cis-mulberroside A isolated from Ramulus mori in several models of inflammatory pain in mice Cis-mulberroside A (25 and 50 mg/kg) given by p o route 30 min before challenge produced a dose-dependent inhibition of the acetic acid-induced pain and Evans blue leakage in mice In addition, this compound exhibited significant systemic anti-inflammatory activity in carrageenan-induced mouse paw edema in a concentration-related manner (33 1 -68 5% inhibition), and similar results were achieved in formalin test Suppressive effects of cis-mulberroside A on the production of NO and expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated macrophages were also assessed Collectively, cis-mulberroside A showed high analgesic and anti-inflammatory activities The above results will be the supporting evidence for the potential anti-rheumatoid activity of R mori in Chinese traditional medicine (C) 2009 Elsevier B V All rights reserved