Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 83, Issue -, Pages 359-367Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2018.09.041
Keywords
Crucian carp; Lactococcus lactis; Aeromonas hydrophila; Immunomodulatory effect; Intestinal mucosal barrier
Funding
- National Natural Science Foundation of China [31372454]
- Three New Aquatic Projects in Jiangsu Province [D2017-3-1]
- Independent Innovation Fund of Agricultural Science and Technology in Jiangsu Province [CX(17)2027]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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This study explored the immunomodulatory effect and inhibition effects of the candidate probiotic Lactococcus lactis 16-7, which was isolated from crucian carp, on Aeromonas hydrophila infection in crucian carp. The experimental fish were divided into two groups; one was fed a diet supplemented with L. lactis, while the other was fed the control probiotic-free diet. After feeding for 42 d with the experimental diets, the fish that received the diet supplemented with probiotics exhibited a significantly enhanced serum superoxide dismutase activity, phagocytic activities of innate immune cells, and the expression levels of immune-related genes [interferon-gamma (INF-gamma), interleukin-1 beta (IL-1 beta), interleukin-11 (IL-11), tumour necrosis factor alpha (TNF-alpha) and myeloid differentiation factor 88 (MyD88)], indicating that L. lactis 16-7 could activate the non-specific immune system of crucian carp. At the end of the feeding trial, the crucian carps in each group were orally infected with A. hydrophila NJ-35. The results show that L. lactic 16-7 could prevent the increase in n-lactic acid concentration and inflammatory response caused by A. hydrophila in crucian carp. Compared with A. hydrophila group, L. lactic 16-7 preserved the integrity of intestinal villi and mitigated A. hydrophila-induced reduce in the transcriptional levels of tight junction (TJ) proteins zonula occludens-1 (ZO-1) and occludin, indicating that L. lactis 16-7 could reduce intestinal mucosal barrier damage and inflammation induced by A. hydrophila in crucian carp. In addition, L. lactis 16-7 could effectively antagonize the colonization of A. hydrophila in the intestine. Overall, these data clearly indicate that L. lactic 16-7 has the potential to be developed as a probiotic agent against A. hydrophila infection in aquaculture.
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