Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 38, Issue 2, Pages 400-405Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2014.03.030
Keywords
Allograft inflammatory factor-1; Apostichopus japonicus; Bacteria challenge; Tissue injury
Funding
- National High Technology Research and Development Program of China [2012AA10A412]
- Key Scientific Specific Project of Liaoning Province, China [2011203003]
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Allograft inflammatory factor-1 (AIF-1) is an interferon (IFN)-y-inducible Ca2+-binding cytokine that associates with the immune defense and inflammatory response. In this study, we reported AlF-1 gene in sea cucumber Apostichopus japonicus (AjAIF-1). The full-length cDNA of AjA1F-1 is 1541 bp with an open reading frame (ORF) of 477 bp encoding 158 amino acids. Two EF-hand Ca2+-binding motifs were found in the deduced AjAIF-1. AjAIF-1 was widely expressed in all tested tissues (body wall, intestine, respiratory tree, tube feet, coelomocytes and longitudinal muscle), with the highest expression in respiratory tree. After Vibrio splendidus challenge and physical injury, AjAIF-1 transcripts were significantly upregulated in coelomocytes. The mRNA expression level of AjA1F-1 in coelomocytes reached to the highest value at 4 h (3.38-folds vs. the PBS control, P < 0.05) post injection. After papilla injury, the mRNA level of AjAIF-1 in coelomocytes was upregulated, and its peak value was found at 4 h (3.88-folds vs. the control, P < 0.05). These results indicated that 1) AjAIF-1 sensitively responds to pathogen infection; 2) AjAIF-1 is involved in acute inflammatory response. Our findings gain general information about the role of AjAIF-1 in the innate immunity of A. japonicus.(C) 2014 Elsevier Ltd. All rights reserved.
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