Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 39, Issue 2, Pages 312-320Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2014.05.026
Keywords
IL-12p40; Oplegnathus fasciatus; Cytokine; Immune defense; Bacterial infection
Funding
- 863 High Technology Project [2011AA10A216]
- Taishan Scholar Program of Shandong Province
Ask authors/readers for more resources
IL-12p40, also called IL-12 beta, is a subunit of the proinflammatory cytokines interleukin (IL)-12 and IL-23. In teleost, IL-12p40 homologues have been identified in several species, however, the biological function of fish IL-12p40 is essentially unknown. In this work, we reported the identification and analysis of an IL-12p40, OflL-12p40, from rock bream (Oplegnathus fasciatus). OflL-12p40 is composed of 361 amino acids and possesses a conserved IL-12p40 domain and a WSxWS signature motif characteristic of known IL-12p40. Constitutive expression of OflL-12p40 occurred in multiple tissues and was highest in kidney. Experimental infection with bacterial pathogen upregulated the expression of OfIL-12p40 in kidney and spleen in a time-dependent manner. Purified recombinant OflL-12p40 (rOflL-12p40) stimulated the respiratory burst activity of peripheral blood leukocytes in a dose-dependent manner. rOflL-12p40 also enhanced the resistance of rock bream against bacterial infection and upregulated the expression of innate immune genes in kidney. Taken together, these results indicate that OflL-12p40 possesses cytokine-like property and plays a role in immune defense against bacterial infection. (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available