Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 35, Issue 3, Pages 988-992Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2013.05.026
Keywords
Brown trout; Gene expression; Innate immunity; Ontogeny; Salmo trutta
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The innate immune system is a fundamental defense weapon of fish, especially during early stages of development when acquired immunity is still far from being completely developed. The present study aims at looking into ontogeny of innate immune system in the brown trout, Salmo trutta, using RT-PCR based approach. Total RNA extracted from unfertilized and fertilized eggs and hatchlings at 0,1 h and 1, 2, 3, 4, 5, 6, 7 weeks post-fertilization was subjected to RT-PCR using self-designed primers to amplify some innate immune relevant genes (TNF-alpha, IL-1 beta, TGF-beta and lysozyme c-type). The constitutive expression of beta-actin was detected in all developmental stages. IL-1 beta and TNF-alpha transcripts were detected from 4 week post-fertilization onwards, whereas TGF-beta transcript was detected only from 7 week post-fertilization onwards. Lysozyme c-type transcript was detected early from unfertilized egg stage onwards. Similarly, tissues such as muscle, ovary, heart, brain, gill, testis, liver, intestine, spleen, skin, posterior kidney, anterior kidney and blood collected from adult brown trout were subjected to detection of all selected genes by RT-PCR. TNF-alpha and lysozyme c-type transcripts were expressed in all tissues. IL-1 beta and TGF-beta transcripts were expressed in all tissues except for the brain and liver, respectively. Taken together, our results show a spatial-temporal expression of some key innate immune-related genes, improving the basic knowledge of the function of innate immune system at early stage of brown trout. (C) 2013 Elsevier Ltd. All rights reserved.
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