Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 34, Issue 2, Pages 724-728Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2012.11.041
Keywords
IgNAR; Neutralization activity; Phage display library; VHSV
Funding
- Ministry of Education, Culture, Sports, Science and Technology, Japan [21248025]
- World Class University Program [R32-10253]
- Korean Ministry of Education, Science, and Technology of South Korea
- Grants-in-Aid for Scientific Research [21248025] Funding Source: KAKEN
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Phage display libraries are used to screen for nucleotide sequences that encode immunoglobulin variable (V) regions that are specific for a target antigen. We previously constructed an immunoglobulin new antigen receptor (IgNAR) phage display library. Here we used this library to obtain an IgNAR V region that is specific for viral hemorrhagic septicemia virus (VHSV). A phage clone (clone 653) was found to be specific for VHSV by the biopanning method. The V region of clone 653 was used to construct a 6 x His tagged recombinant IgNAR-653 V protein (rIgNAR-653) using the Escherichia coli pET system. The rIgNAR-653 protein bound specifically to VHSV, confirming its activity. (C) 2012 Elsevier Ltd. All rights reserved.
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