Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 34, Issue 6, Pages 1416-1420Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2013.02.023
Keywords
Enzymatic hydrolysis; TFF system; Mytilus coruscus; Anti-inflammatory peptide
Funding
- Ministry for Food, Agriculture, Forestry and Fisheries
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Shellfish contain significant levels of high quality protein and are therefore a potential source for bio-functional high-value peptides. To purify a novel anti-inflammatory peptide from Mytilus coruscus (M. coruscus), we applied enzymatic hydrolysis and tangential flow filtration (TFF) and investigated its nitric oxide inhibitory property. To prepare the peptide, eight proteases were employed for enzymatic hydrolysis. Flavouzyme hydrolysates, which showed clearly superior nitric oxide inhibitory activity on lipopolysaccharide (LPS)-stimulated RAW264.7, were further purified using a TFF system and consecutive chromatographic methods. Finally, a novel anti-inflammatory peptide composed of 10 amino acid residues was obtained, and the sequence was identified as Gly-Val-Ser-Leu-Leu-Gln-Gln-Phe-Phe-Leu at N-terminal position. The peptide from M. coruscus effectively inhibited nitric oxide production on macrophage cells. This is the first report of an anti-inflammatory peptide derived from the hydrolysates of M. coruscus. (C) 2013 Elsevier Ltd. All rights reserved.
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