Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 32, Issue 1, Pages 45-50Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2011.10.017
Keywords
Antifungal peptide; Q-PCR; Innate immunity; Challenge; Specificity
Funding
- EU [KBBE-2010-266157]
- PICS CNRS [5197]
- University of Palermo-Italy
- Italian Ministry of Education
- Burapha University from Thailand
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We previously reported the nucleotide sequences and diversity of mytimycin (MytM) from the Mediterranean mussel, Mytilus galloprovincialis. Using real-time PCR (q-PCR), we observed that the MytM gene was mainly expressed in circulating hemocytes and to a less extent in the mantle. In vivo challenge with bacteria or with the yeast. Candida albicans, did not increase the expression as measured by q-PCR in hemocytes. By contrast, injection of the filamentous fungus, Fusarium oxysporum, induced a sudden and strong increase of expression at 9h p.i. (stimulation index of 25.7 +/- 2.1). Optimum stimulating dose was 10(4) spores of F. oxysporum per mussel. In the same samples, AMP mytilin and myticin showed no stimulation. Consequently, we hypothesized the existence of 2 different signal transduction pathways, one activated by bacteria and yeast, the other triggered by filamentous fungi. A second challenge performed with F. oxysporum 24 h after the first challenge induced an increase of MytM gene expression (stimulation index of 3.5 +/- 1.7). However, this second increase was significantly lower than the first, suggesting less efficient response rather than significant protection. (C) 2011 Elsevier Ltd. All rights reserved.
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