Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 31, Issue 6, Pages 838-847Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2011.07.024
Keywords
Bacterial challenge; Immune-related genes; Nitric oxide; Respiratory burst activity; Stress response
Funding
- Spanish Ministerio de Ciencia e Innovacion [CSD2007-00002]
- ANYFISH
- PO Algarve 21
- QREN
- FEDER (European Union)
- Fundacao para a Ciencia e a Tecnologia, Portugal [SFRH/BD/38697/2007]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/38697/2007] Funding Source: FCT
Ask authors/readers for more resources
Stress is known to impair immune function and disease resistance in fish. In the present study, repeated handling was employed as a chronic stressor in order to verify whether its attributed immunosuppressive effects could be minimized by dietary arginine supplementation. Therefore, Senegalese sole (Solea senegalensis) were air exposed daily for 3 min during 14 days (handling) or left undisturbed (control). In addition, both control and handled specimens were fed 3 diets with graded levels of arginine (Arg 4.4, Arg 5.7 and Arg 6.9 g 16 g(-1) N). Following the 14 days stress challenge and feeding on those diets, fish were infected with Photobacterium damselae subsp. piscicida (strain PC566.1; LD(50)5 x 10(3) cfu mL(-1)) and fed the same experimental diets. Respiratory burst activity and nitric oxide production of head-kidney leucocytes increased parallel to dietary arginine supplementation. HIF-1, HAMP-1, MIP1-alpha and gLYS expression values and some humoral parameters augmented in control specimens fed the Arg 5.7 and Arg 6.9 diets. Interestingly, repeated acute stress increased both disease resistance and some innate immune mechanisms in handled fish. The role of dietary arginine and repeated handling on Senegalese sole innate immunity and disease resistance are discussed. (C) 2011 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available