Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 31, Issue 4, Pages 524-532Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2011.06.015
Keywords
Paralichthys olivaceus; Suppression subtractive hybridization (SSH); Microarray; Edwardsiella tarda; Viral hemorrhagic septicemia virus (VHSV)
Funding
- Ministry of Agriculture, Forestry and Fisheries of Japan
- Ministry of Education, Science, Sports and Culture of Japan
- Grants-in-Aid for Scientific Research [21380126, 22380112] Funding Source: KAKEN
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Transcriptional changes in the peripheral blood leucocytes (PBL) of Japanese flounder Paralichthys olivaceus challenged by Edwardsiella tarda and viral hemorrhagic septicemia virus (VHSV) were investigated using suppression subtractive hybridization (SSH) coupled with cDNA microarray analysis. First, we constructed an SSH cDNA library using mRNA samples isolated from PBL of P. olivaceus that had been experimentally infected with E. tarda. We then examined the transcriptional changes occurring in the PBL due to E. tarda and VHSV infection using a cDNA microarray produced using clones produced from the SSH library. A total of 565 and 180 cDNA sequences corresponding to mRNA species that are either up- or down-regulated by E. tarda infection were isolated by SSH. While host gene expression responses in response to E. tarda and VHSV infection share several response elements, distinct patterns of gene expression were also observed. specifically, E. tarda infection enhanced the expression of cell adhesion molecules while VHSV enhanced the expression of interferon and proteasome-related genes. In challenge trials of the two infectious agents, expression profiles of chemokines were also observed to differ. The results indicated that distinguishing between viral and bacterial infection is possible based on the RNA expression profiles of PBL from infected fish. (C) 2011 Elsevier Ltd. All rights reserved.
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