Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 25, Issue 1-2, Pages 188-190Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2007.12.006
Keywords
elasmobranch; little skate; Leucoraja erinacea; leucocytes; erythrocytes; density gradient centrifugation
Funding
- NCRR NIH HHS [R01-RR-019732, RR016463, R01 RR019732-03, R01 RR019732, P20 RR016463, P20 RR016463-08] Funding Source: Medline
- NIEHS NIH HHS [ES03828, P30 ES003828-23, P30 ES003828] Funding Source: Medline
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Cartilaginous fish, especially sharks, rays and skates (elasmobranchs), hold interest as comparative models in immunology because they are thought to be among the organisms most closely related to the ancestor animal that first developed acquired immunity. The aim of this study was to improve methods used for the purification of viable leucocytes from peripheral blood of elasmobranchs. Here we describe modifications of density gradient centrifugation and medium formulation that improve isolation and analysis of highly purified leucocytes from peripheral blood of a model elasmobranch, Leucoraja erinacea, the little skate. These techniques contribute to the preparation of elasmobranch immune cells that can be reliably analyzed by a variety of means, including the study of immune function. (C) 2007 Elsevier Ltd. All rights reserved.
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