Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 25, Issue 1-2, Pages 76-83Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2007.12.013
Keywords
carp; skin; Argulus; cytokine; chemokine; ectoparasite; immune response
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In the present study we investigated changes in transcription levels of a panel. of selected immune relevant genes in peripheral blood leucocytes (PBL) and skin samples collected from carp exposed to larval Argulus japonicus. We show that in skin up-regulation of gene transcription of the chemokine CXCa, and to a lesser extent the chemokine receptor CXCR1 and the cytokine TNF alpha, are good indicators of parasite-induced skin damage at 2 days post-parasite exposure. Up-regulation of gene transcription corresponded well. with an increase in leucocytes, probably neutrophilic granulocyte numbers in skin samples collected at the sites of infection. We show that time-point controls are essential. when studying gene expression, especially in peripheral blood leucocytes (PBL). In addition, we demonstrate that non-infected control samples isolated from the skin of infected fish are suitable autologous controls, at least until after larval A. joponicus have undergone their first moult and begun to demonstrate increased mobility over their host's integument. The observed results are indicative of A. japonicus affecting the skin as a whole organ, particularly after the parasites' first moult, a phenomenon which has a great impact on correct skin sampling for RNA isolation. (C) 2008 Elsevier Ltd. All rights reserved.
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